frequency of null allele of human leukocyte antigen-g (hla-g) locus in subjects to recurrent miscarriage
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abstract
background: human leukocyte antigen-g (hla-g) is a non-classical class i molecule highly expressed by extravillous cytotrophoblast cells. due to a single base pair deletion, its function can be compensated by other isoforms. investigating the frequency of null allele in recurrent miscarriage (rm) subjects could be useful in understanding the relationship between frequency of this allele and rm in a given population. objective: this study aimed to determine the frequency of hla-g*0105n null allele and its potential association with down-regulation of hla-g in subjects with rm. materials and methods: western blotting was used to assess the level of hla-g protein expression. for investigating the frequency of hla-g*0105n null allele in rm subjects, pcr-rflp method was used. exon 3 of hla-g gene was amplified by polymerase chain reaction (pcr). subsequently, ppum-1 enzyme was employed to digest the pcr products and fragments were analyzed using gel electrophoresis. results: digestion using restriction enzyme showed the presence of heterozygous hla-g*0105n null allele in 10% of the test population. western blotting results confirmed the decrease in expression of hla-g in the placental tissue of subjects with rm compared to subjects who could give normal birth. conclusion: the frequency of heterozygous hla-g*0105n null allele was high to some extent in subjects with rm. the mutation rate in subjects suggested that there is a significant association between rm and frequency of mutations in this allele.
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Frequency of null allele of Human Leukocyte Antigen-G (HLA-G) locus in subjects to recurrent miscarriage
BACKGROUND Human leukocyte antigen-G (HLA-G) is a non-classical class I molecule highly expressed by extravillous cytotrophoblast cells. Due to a single base pair deletion, its function can be compensated by other isoforms. Investigating the frequency of null allele in Recurrent Miscarriage (RM) subjects could be useful in understanding the relationship between frequency of this allele and RM i...
full textNull Allele Frequencies at HLA-G Locus in Iranian Healthy Subjects
Background: HLA-G gene contains 15 alleles including a null allele, HLA-G*0105N. Previous studies have shown that HLA-G*0105N does not encode the complete HLA-G1 or HLA-G5 isoforms but encodes a functional HLA-G protein with the ability to in-hibit NK cell cytolysis. Thus, although the biological functions of HLA-G1 and HLA-G5 proteins are abrogated, other isoforms such as HLA-G2 can replace th...
full textnull allele frequencies at hla-g locus in iranian healthy subjects
background: hla-g gene contains 15 alleles including a null allele, hla-g*0105n. previous studies have shown that hla-g*0105n does not encode the complete hla-g1 or hla-g5 isoforms but encodes a functional hla-g protein with the ability to in-hibit nk cell cytolysis. thus, although the biological functions of hla-g1 and hla-g5 proteins are abrogated, other isoforms such as hla-g2 can replace th...
full textHLA-G*0105N null allele encodes functional HLA-G isoforms.
Expression of the nonclassical HLA class I antigen, HLA-G, is associated with immune tolerance in view of its role in maintaining the fetus in utero, allowing tumor escape, and favoring graft acceptance. Expressed on invasive trophoblast cells, HLA-G molecules bind inhibitory receptors on maternal T lymphocytes and NK cells, thereby blocking their cytolytic activities and protecting the fetus f...
full textNull allele frequencies at HLA-G locus in Iranian healthy subjects.
BACKGROUND HLA-G gene contains 15 alleles including a null allele, HLA-G*0105N. Previous studies have shown that HLA-G*0105N does not encode the complete HLA-G1 or HLA-G5 isoforms but encodes a functional HLA-G protein with the ability to inhibit NK cell cytolysis. Thus, although the biological functions of HLA-G1 and HLA-G5 proteins are abrogated, other isoforms such as HLA-G2 can replace thei...
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Journal title:
international journal of reproductive biomedicineجلد ۱۴، شماره ۷، صفحات ۴۵۹-۰
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